A database about Preparation of Laboratory Reagents. A Project of SCIENCE Pakistan


Chloroform, CHCl3

DANGER! Suspected carcinogen. WARNING! Harmful if
inhaled. Can cause embryo-fetal injury Avoid breathing
vapor. Keep container closed Use with adequate ventilation.
Avoid prolonged or repeated contact with skin.

Washed Chloroform
  1. From a graduated cylinder, add 250 mL of spectro
    grade chloroform to a separatory funnel.
  2. Add 125 mL of distilled water from a graduate
  3. Stopper and shake the funnel for 30 seconds.
  4. Allow the layers to separate for 10 minutes before
    decanting the chloroform
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