• Citric Acid 1-Hydrate, HOC(COOH)(CH2COOH)2•H2O
• Sodium Citrate 2-Hydrate,
NaOC(COOH)(CH2COONa)2•2H2O
Warning
Causes eye burns and skin irritation. Do not get in eyes, on
skin, on clothing. Wear impervious gloves and eye
protection when using.
Preparation
2 M Citrate Buffer
• Sodium Citrate 2-Hydrate,
NaOC(COOH)(CH2COONa)2•2H2O
Warning
Causes eye burns and skin irritation. Do not get in eyes, on
skin, on clothing. Wear impervious gloves and eye
protection when using.
Preparation
2 M Citrate Buffer
- Add approximately 600 mL of distilled water to a
1-litre volumetric flask on a magnetic stirrer. - Add and dissolve 210.14 g of citric acid 1-hydrate.
- Add and dissolve 294.11 g of sodium citrate
2-hydrate. - Dilute to volume with distilled water.
- The pH of this solution is 3.92 ± 0.03 at 77°F (25°C).
If not, adjust this solution by adding small amount of
sodium hydroxide to raise the pH or citric acid to
lower the pH.
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