A database about Preparation of Laboratory Reagents. A Project of SCIENCE Pakistan

Ethyl Acetate

Ethyl acetate, CH3COOCH2CH3

FLAMMABLE. Keep away from heat, sparks and flame.
Avoid breathing vapor. Keep container closed. Use with
adequate ventilation. Avoid prolonged or repeated contact
with skin.

Absorbance Check
When ethyl acetate is used in a spectrophotometric method,
check it for ultraviolet absorbance before it is used. If the
absorbance of a 1 cm silica cell filled with ethyl acetate
exceeds 0.150, measured against an air blank at 295 and
315 nm, the ethyl acetate is not suitable for use.


Note: If large volumes of these reagents are needed, a large
mixing container may be used. If a power stirrer is used, it
must be air-driven or electrically driven (sparkless) to avoid
the possibility of a spark igniting the flammable vapor.
Water-Saturated Ethyl Acetate

Note: Anhydrous ethyl acetate absorbs water very rapidly.
Variations in its water content produce changes in volume
when it is shaken with an aqueous sample. Since ethyl
acetate cannot be assumed to be anhydrous, and since the
water content is unimportant, provided it is always the same
from one batch to another, it is recommended that it be
saturated with water prior to use (3.3 mL of water will
dissolve in 90 mL of anhydrous ethyl acetate).
  1. Add 100 mL of distilled water to 900 mL of reagent (or spectro-reagent) grade ethyl acetate.
  2. Mix well in a container large enough to allow for expansion.
  3. Decant the water-saturated ethyl acetate or withdraw the lower (aqueous) layer by using a length of glass tubing attached to an aspirator pump.

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